Deep Mutagenesis of a DNA Polymerase Clamp-Loader System
Clamp loaders are AAA+ ATPases that load sliding clamps onto DNA. We have developed a high-throughput platform for mutagenesis of components of the DNA replication system of T4 bacteriophage, and are using it to understand allosteric coupling in the component proteins.
Speaker: John Kuriyan, UC Berkeley
Monday, 08/30/21
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